anti fatp1  (R&D Systems)

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Cryoshocked Adipocytes Mediated Dual-Modal Strategy Combining Photodynamic Therapy and Triptolide Palmitate for Pulmonary Metastatic Melanoma Treatment.

doi: 10.1002/advs.202414307

Figure Lengend Snippet: Figure 2. Cellular uptake of Ce6-pTP-CsA. a) Representative CLSM images and f) analysis of MFI showing the cellular uptake of BODIPY-stained Ce6- pTP-CsA co-incubated with A375-M1 cells for 12 h, 24 h, and 48 h (n = 3). Scale bar: 50 μm. b,c) Western blot analysis of FATP1, FABP4, and CD36 expression in A375-M1 cells treated with PBS, PA, pTP, and Ce6-pTP-CsA (n = 3). d) CLSM and g) MFI analysis of Ce6 uptake in A375-M1 cells for 3, 12, and 24 h (n = 3). Scale bar: 20 μm (n = 3). e) FCM analysis of Ce6 uptake in A375-M1 cells for different durations (n = 3). (ns, not significant; **p < 0.01; ***p < 0.001; ****p < 0.0001).

Article Snippet: The antibodies used for Western blotting, including FATP1, FABP4, CD36, CHOP, and ATF4, were obtained from Proteintech (IL, USA); P-elF2α and Bcl-2 primary antibodies were from Abclonal (MA, USA); the PERK primary antibody was from Servicebio (Wuhan, China); and the Cleaved PARP, Cleaved-Caspase 3, and CleavedCaspase 9 primary antibodies were from CST (MA, USA).

Techniques: Staining, Incubation, Western Blot, Expressing

Journal: Journal of Animal Science and Biotechnology

Article Title: Dietary supplementation with pterostilbene activates the PI3K-AKT-mTOR signalling pathway to alleviate progressive oxidative stress and promote placental nutrient transport

doi: 10.1186/s40104-024-01090-9

Figure Lengend Snippet: Effect of PTE on placental nutrient transport capacity. A – C Fatty acid transporter FATP1 and glucose transporter GLUT3, 200× analysis of IF staining. Data are expressed as mean ± SD ( n = 3 for each group). * P < 0.05, compared to the control group

Article Snippet: Anti-FATP1 antibody (Abclonal WH342075) was purchased from Abclonal (Wuhan, China).

Techniques: Staining, Control

Journal: Frontiers in molecular biosciences

Article Title: RabGAP AS160/TBC1D4 deficiency increases long-chain fatty acid transport but has little additional effect on obesity and metabolic syndrome in ADMSCs-derived adipocytes of morbidly obese women.

doi: 10.3389/fmolb.2023.1232159

Figure Lengend Snippet: FIGURE 3 Transcript level of fatty acid transporters, i.e., CD36/SR-B2 (A), FATP1 (B), and FATP4 (C) in AS160 knocked down mature adipocytes differentiated from ADMSCs of patients with different metabolic status. Data are presented as mean ± SD. Values are expressed in arbitrary units, i.e., lean non-targeting siRNA group was set at 1 [AU]. # Difference between the cells transfected with siRNA targeting AS160 and scrambled siRNA (negative control) within the metabolic status of the donor patient; p < 0.05; n = 4 for each study group (measurements taken in duplicate). Designations of the groups: NC, negative control containing non-targeting siRNA fragment; siAS160, knockdown of AS160; obese (−), obese without metabolic syndrome; and obese (+), obese with metabolic syndrome.

Article Snippet: The acquired ADMSCs were subjected to staining with monoclonal antibodies targeting anti-FATP1 (ACSVL5; mouse anti-human) (R&D Systems), anti-FATP4 (ACSVL4; rabbit anti-human), and anti-CD36/SR-B2 (CD36/SR-B2; rabbit antihuman) (Abcam).

Techniques: Transfection, Negative Control, Knockdown

Journal: Frontiers in molecular biosciences

Article Title: RabGAP AS160/TBC1D4 deficiency increases long-chain fatty acid transport but has little additional effect on obesity and metabolic syndrome in ADMSCs-derived adipocytes of morbidly obese women.

doi: 10.3389/fmolb.2023.1232159

Figure Lengend Snippet: FIGURE 4 Total protein abundance of fatty acid transporters, i.e., CD36/SR-B2 (A), FATP1 (B), and FATP4 (C) in AS160 knocked down mature adipocytes differentiated from ADMSCs of patients with different metabolic status. Data are presented as mean ± SD. # Difference between cells transfected with siRNA targeting AS160 and scrambled siRNA (negative control) within the metabolic status of the donor patient; p < 0.05; n = 4 for each study group (measurements taken in duplicate). Designations of the groups: NC, negative control containing non-targeting siRNA fragment; siAS160, knockdown of AS160; obese (−), obese without metabolic syndrome; and obese (+), obese with metabolic syndrome.

Article Snippet: The acquired ADMSCs were subjected to staining with monoclonal antibodies targeting anti-FATP1 (ACSVL5; mouse anti-human) (R&D Systems), anti-FATP4 (ACSVL4; rabbit anti-human), and anti-CD36/SR-B2 (CD36/SR-B2; rabbit antihuman) (Abcam).

Techniques: Quantitative Proteomics, Transfection, Negative Control, Knockdown

Journal: Frontiers in molecular biosciences

Article Title: RabGAP AS160/TBC1D4 deficiency increases long-chain fatty acid transport but has little additional effect on obesity and metabolic syndrome in ADMSCs-derived adipocytes of morbidly obese women.

doi: 10.3389/fmolb.2023.1232159

Figure Lengend Snippet: FIGURE 5 Plasma membrane abundance of fatty acid transporters, i.e., CD36/SR-B2 (A), FATP1 (B), and FATP4 (C) in AS160 knocked down mature adipocytes differentiated from ADMSCs of patients with different metabolic status evaluated by flow cytometry analysis. Data are presented as mean ± SD. # Difference between the cells transfected with siRNA targeting AS160 and scrambled siRNA (negative control) within the metabolic status of the donor patient; p < 0.05; n = 4 for each study group (measurements taken in duplicate). Designations of the groups: NC, negative control containing non- targeting siRNA fragment; siAS160, knockdown of AS160; obese (−), obese without metabolic syndrome; and obese (+), obese with metabolic syndrome.

Article Snippet: The acquired ADMSCs were subjected to staining with monoclonal antibodies targeting anti-FATP1 (ACSVL5; mouse anti-human) (R&D Systems), anti-FATP4 (ACSVL4; rabbit anti-human), and anti-CD36/SR-B2 (CD36/SR-B2; rabbit antihuman) (Abcam).

Techniques: Clinical Proteomics, Membrane, Cytometry, Transfection, Negative Control, Knockdown